Two research projects focusing on support limb laminitis have received funding from the American Association of Equine Practitioners (AAEP) Foundation.
AAEP members have identified laminitis as the most important equine disease requiring research. Support limb laminitis takes place when lameness in one limb results in laminitis in the opposite limb. Subsequent separation of the coffin bone from the hoof wall causes pain and eventually displacement, either coffin bone rotation or sinking, in the foot. Support limb laminitis was the type of laminitis that 2006 Kentucky Derby winner Barbaro succumbed to while healing from a severe fracture in a hind limb.
“The generosity of many enabled us to fund these projects that will advance the knowledge and help unravel the mysteries surrounding this insidious disease,” says veterinarian Jeff Berk, chairman of the AAEP Foundation Advisory Council. “We are particularly grateful to Starlight Racing partners, whose matching funds challenge in 2014 in memory of its Grade II winner Intense Holiday raised awareness and much-needed funds for the fight.”
University of Pennsylvania veterinarian Hana Galantino-Homer will investigate the alterations that occur in the protein structure that supports the bone to the hoof connection. Foot specimens from horses affected by support limb laminitis will be used to study how nutrient deprivation and subsequent production of abnormal proteins (keratins) affect the support structures within the hoof. The goal is to understand the process of cell pathology in the foot to better predict, diagnose and treat support limb laminitis.
The second study, led by Samantha Brooks at the University of Florida, seeks to understand the response of the cells in the support structures within the hoof. By utilizing RNA sequencing, genes that respond to the abnormal support in the foot will be identified and compared with normal feet. Understanding the gene upregulation will help identify the process within the hoof that leads to support failure. The research will use real-time PCR to identify the production of inflammatory mediators and enzymes involved with the pathology.
Both studies will utilize the Laminitis Discovery Database at the University of Pennsylvania, which has specimens from horses that succumbed to support limb laminitis and from normal horses. The database includes a wealth of information about the affected horses.